Isoforms of Human Recombinant Follicle-Stimulating Hormone: Comparison of Effects on Murine Follicle Development In Vitro1

Effects of recombinant human follicle-stimulating hormone on embryo development in mice.

We have developed a protocol using recombinant human follicle-stimulating hormone (rhFSH) to induce ovarian stimulation in the mouse to investigate its impact on preimplantation embryo development. Embryos were collected from adult female C57Bl/6 x CBA F1 mice treated with rhFSH (0, 2.5, 5.0, 10.0, or 20.0 IU) or 5 IU equine chorionic gonadotropin (eCG). Embryos were also recovered from nontrea...

Dissociation of human follicle-stimulating hormone. Comparison with luteinizing hormone.

Rat testis tissue receptor assays were utilized to study the kinetics of dissociation of human follicle-stimulating hormone (hFSH) and luteinizing hormone (hLH) under varying conditions of urea concentration and pH. In these competitive protein binding assays, 125I-hFSH and 125I-hLH were the radioligands and hormone dissociation was followed by a decrease in the ability of the dissociating horm...

The Correlations of Anti-Mullerian Hormone, Follicle-Stimulating Hormone and Antral Follicle Count in Different Age Groups of Infertile Women

Overnight radioimmunoassay of human follicle-stimulating hormone.

A radioimmunoassay procedure for follicle-stimulating hormone in serum has been developed in which the assay mixture is incubated at 37#{176}C for a total of 22 h, rather than at 4#{176}C for 4-7 days. The withinassay coefficients of variation for the 37#{176}C and 4#{176}C methods, respectively, are 7.7% and 5.0%, while the between-assay variations are 13.8% and 9.9%. Comparison of values for ...

Meta-analysis on recombinant versus urinary follicle stimulating hormone.

A recent meta-analysis (Daya and Gunby, 1999) on recombin-ant versus urinary follicle stimulating hormone (rFSH versus uFSH) deserves methodological comment. The a priori definition of eligibility criteria for studies to be included in a meta-analysis are central and must be systematic. It was up to the authors to decide whether their search would be extended to include all unpublished studies,...